abstract：:This study investigated time-dependent variations in the activities of adenosine deaminase (ADA), an adenosine-metabolizing enzyme, and myeloperoxidase (MPO), an oxidation reaction-catalyzing enzyme, in control and streptozotocin (STZ)-induced diabetic rat liver. The animals were sacrificed at six different times of d...

journal_title：General physiology and biophysics

authors： Uluoglu C,Cimen B,Ozbey G,Karasu C,Durakoglugil DB,Gunes A,Turkozkan N,Zengil H

更新日期：2008-03-01 00:00:00

abstract：:Elevated serum resistin is implicated in insulin resistance associated with obesity and type 2 diabetes mellitus. Alcohol consumption interferes with the nutritional status, metabolic and hormonal activity of the drinker. Impact of ethanol intake on resistin level and resistin metabolic effects is unknown. Effect of l...

journal_title：General physiology and biophysics

authors： Pravdová E,Macho L,Hlavácová N,Ficková M

更新日期：2007-09-01 00:00:00

abstract：:Pretreatment with diazoxide, mitochondrial K(ATP) channel opener, was found to protect the rat heart against ischemia/reperfusion injury. Our aim was also to characterize the effects of diazoxide on the alterations of regulatory myocardial proteins, on mitochondrial ultrastructure, integrity and induction of apoptotic...

journal_title：General physiology and biophysics

authors： Simoncíková P,Ravingerová T,Andelová E,Tribulová N,Barancík M

更新日期：2007-06-01 00:00:00

abstract：:The effects of different parts of extract from medicinal plant Conyza canadensis, used to control bleeding, on human blood platelet aggregation in vitro were investigated. Aqueous extract of Conyza c. from young or old plants, glycoconjugate part, polysaccharide part and aglycon part at the concentrations above 0.75 m...

journal_title：General physiology and biophysics

authors： Saluk-Juszczak J,Olas B,Pawlaczyk I,Gancarz R,Wachowicz B

更新日期：2007-06-01 00:00:00

abstract：:Lidocaine, a use-dependent Na(+) channel blocker, paradoxically evokes neural activation in the slowly adapting stretch receptor organ of crayfish at 5-10 mmol/l concentration. For elucidating the underlying mechanisms of this paradoxical effect, a series of conventional electrophysiological experiments were performed...

journal_title：General physiology and biophysics

authors： Keceli MB,Purali N

更新日期：2007-03-01 00:00:00

abstract：:The role of C-type natriuretic peptide (CNP) in the gastrointestinal tract is still unclear. This study was designed to investigate the effect of CNP on barium current (I(Ba)) through the L-type calcium channel in gastric antral myocytes of guinea pigs. The whole-cell patch clamp technique was performed in gastric ant...

journal_title：General physiology and biophysics

authors： Sun JB,Huang X,Xu HY,Li XL,Gao L,Kim YC,Xu WX

更新日期：2006-12-01 00:00:00

abstract：:The changes in mitochondrial membrane potential (Deltapsi(m)) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncou...

journal_title：General physiology and biophysics

authors： Lábajová A,Kofránek J,Kriváková P,Cervinková Z,Drahota Z

更新日期：2006-09-01 00:00:00

abstract：:Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...

journal_title：General physiology and biophysics

authors： Neacsu I,Creanga DE,Tufescu FM

更新日期：2006-06-01 00:00:00

abstract：:Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intra...

journal_title：General physiology and biophysics

authors： Ham D,Karska-Wysocki B

更新日期：2005-12-01 00:00:00

abstract：:The effect of melatonin on reperfusion arrhythmias and postischemic contractile dysfunction was studied in the isolated rat heart. 25 min global ischemia was induced and followed by 30 min of reperfusion. Melatonin (10 micromol/l) was present in the perfusion solution during the whole experiment. Experiment revealed p...

journal_title：General physiology and biophysics

authors： Vazan R,Pancza D,Béder I,Styk J

更新日期：2005-09-01 00:00:00

abstract：:Participation of electrostatic and other noncovalent interactions in the binding of tricyclic antidepressants (TCAs) to the lipid bilayers was estimated from pH-dependencies of imipramine, desipramine, amitriptyline and nortriptyline binding to the lipid bilayers prepared from different phospholipids, both electroneut...

journal_title：General physiology and biophysics

authors： Fisar Z

更新日期：2005-06-01 00:00:00

abstract：:Voltage-activated calcium channels can be divided into two subgroups based on their activation threshold, low-voltage-activated (LVA) and high-voltage-activated (HVA). Auxiliary subunits of the HVA calcium channels contribute significantly to biophysical properties of the channels. We have cloned and characterized mem...

journal_title：General physiology and biophysics

authors： Lacinová L

更新日期：2005-06-01 00:00:00

abstract：:In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/abse...

journal_title：General physiology and biophysics

authors： Grdović N,Vidaković M,Poznanović G

更新日期：2005-03-01 00:00:00

abstract：:A growing body of evidence supports the role of free radicals in triggering the functional and metabolic disturbances following transient cerebral ischemia. This study was designed to evaluate whether the extent of reperfusion-induced inhibition of protein synthesis initiation as well as tissue injury can be reduced b...

journal_title：General physiology and biophysics

authors： Hrehorovská M,Burda J,Domoráková I,Mechírová E

更新日期：2004-12-01 00:00:00

abstract：:The effect of hydroxyl radicals generated by the FeSO4/H2O2 system on structural properties of proteins and membranes was studied in rat cardiac mitochondria and myofibrils. Exposure of mitochondria to 0.1 mmol/l FeSO4/EDTA plus 1 mmol/l H202 at 37 degrees C for 30 or 60 min caused conjugated diene formation, but it w...

journal_title：General physiology and biophysics

authors： Babusíková E,Kaplán P,Lehotský J,Jesenák M,Dobrota D

更新日期：2004-09-01 00:00:00

abstract：:Homeostasis and development in vertebrates are regulated by cell proliferation, differentiation and death. Permeability of mitochondrial membranes, a decisive feature of apoptosis, is regulated by Bcl-2 family regulators. Protein p53 is able to reduce bcl-2 and promote bax expression. This study focused on the immunoh...

journal_title：General physiology and biophysics

authors： Prochazkova J,Lichnovsky V,Kylarova D,Erdosova B,Vranka P

更新日期：2004-06-01 00:00:00

abstract：:Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms wer...

journal_title：General physiology and biophysics

authors： Fisar Z,Fuksová K,Velenovská M

更新日期：2004-03-01 00:00:00

abstract：:The postnatal developmental changes in mitochondrial uncoupling protein 1 (UCP 1) and F1-ATP synthase (ATPase) subunit levels in the interscapular brown adipose tissue (BAT) were studied in golden Syrian hamsters (Mesocricetus auratus) using electron microscopy in situ immunocytochemistry. The relatively low initial d...

journal_title：General physiology and biophysics

authors： Bednár J,Soukup T

更新日期：2003-12-01 00:00:00

abstract：:The aim of this paper was to study the protein remodelling of the left ventricle following repeated administration of either daunorubicin (DNR) or DNR in combination with the cardioprotective agent dexrazoxane (DXZ). The experiment was carried out on three groups of Chinchilla male rabbits: 1. DNR (3 mg/kg i.v.), 2. D...

journal_title：General physiology and biophysics

authors： Adamcová M,Pelouch V,Gersl V,Kaplanová J,Mazurová Y,Simůnek T,Klimtová I,Hrdina R

更新日期：2003-09-01 00:00:00

abstract：:CCAAT/enhancer binding proteins (C/EBP) alpha, -beta and -delta play an important role in mediating I interleukin-6 (IL-6) dependent expression of acute-phase protein (APP) genes in liver during acute-phase (AP) response. Based on the presence of type IL-6 responsive element (IL-6 RE) in the rat haptoglobin (Hp) gene ...

journal_title：General physiology and biophysics

authors： Milosavljević T,Lazić T,Uskoković A,Petrović M,Grigorov I

更新日期：2003-06-01 00:00:00

abstract：UNLABELLED:The effect of melatonin (MLT) on the isolated rat heart was studied using the standard perfusion conditions (Langendorff preparation) and model of calcium paradox (Ca(2+)-paradox). Ca(2+)-paradox was induced by 1 minute perfusion with Ca(2+)-free Krebs-Henseleit (KH) solution and subsequent 20 minutes perfus...

journal_title：General physiology and biophysics

authors： Vazan R,Styk J,Béder I,Pancza D

更新日期：2003-03-01 00:00:00

abstract：:The aim of the study was to compare the effect of hemophane and polysulfone membranes on the phagocyte-derived production of reactive oxygen species (ROS) as well as on neutrophil CD11b and CD62L expression in patients undergoing regular hemodialysis. The effects of hemodialysis membranes were also studied in in vitro...

journal_title：General physiology and biophysics

authors： Kubala L,Cíz M,Soska V,Cerný J,Lojek A

更新日期：2002-12-01 00:00:00

abstract：:Malate dehydrogenases (MDH, L-malate:NAD oxidoreductase, EC 1.1.1.37), catalyze the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate/aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondria...

journal_title：General physiology and biophysics

authors： Minárik P,Tomásková N,Kollárová M,Antalík M

更新日期：2002-09-01 00:00:00

abstract：:The effects of pentoxifylline (PTX) on intracellular accumulation of doxorobicin (DOX), DOX cytotoxicity and expression of Pgp in multidrug resistant L1210/VCR cell line were investigated. PTX (100 mg/l) was able to enhance the DOX accumulation in resistant cells. The maximum intracellular levels of DOX were reached a...

journal_title：General physiology and biophysics

authors： Drobná Z,Stein U,Walther W,Barancík M,Breier A

更新日期：2002-03-01 00:00:00

abstract：:Expression of the haptoglobin (Hp) gene is liver specific and acute phase (AP) responsive. It was previously shown that transcriptional induction process of the rat Hp gene during turpentine induced AP response has been mediated by the liver nucleoprotein p29 which was shown to be homologous to the HMG-1 chromatin-ass...

journal_title：General physiology and biophysics

authors： Grigorov I,Milosavljević T,Cvetković I,Petrović M

更新日期：2001-12-01 00:00:00

abstract：:The paper deals with mathematical modelling of the transients obtained by fitting of delayed fluorescence (DF) induction trace. The transients are in certain, doubtless connection with electrochemical gradient (ECG) formed across thylakoid membranes upon illumination. The fitting of the C and D transients by using con...

journal_title：General physiology and biophysics

authors： Marković DZ,Kalauzi A,Radenović CN

更新日期：2001-09-01 00:00:00

abstract：:Aiming at making the Münch model more adequate to the biological reality we introduce certain modifications and complements. Considering the model within the framework of so-called integrated system of long-distance water transport in plants we present a quantitative analysis based on the Kedem-Katchalsky formalism. A...

journal_title：General physiology and biophysics

authors： Kargol M,Suchanek G,Kargol A

更新日期：2001-06-01 00:00:00

abstract：:Fractal analysis was applied to images of photochemical lignin polymer obtained using scanning tunneling microscope. We studied the polymer obtained in vitro by ionic mechanism through UV radiation--induced polymerization. The analysis showed the regularity of the lignin-like polymer at different levels of organizatio...

journal_title：General physiology and biophysics

authors： Radotić K,Tasić M,Jeremić M,Budimlija Z,Simić-Krstić J,Polzović A,Bozović Z

更新日期：2001-03-01 00:00:00

abstract：:Salts of divalent cadmium are well-known human mutagens and carcinogens. In the present work, the ability of vitamin C to modulate genotoxic effects of cadmium chloride on human lymphocytes was assessed using single cell gel electrophoresis (comet assay). Vitamin C at 20 and 100 micromol/l and cadmium at 5, 30 and 150...

journal_title：General physiology and biophysics

authors： Błasiak J,Trzeciak A,Dziki A,Ulańska J,Pander B

更新日期：2000-12-01 00:00:00

abstract：:Fasting for 48 h and the same period of recovery induced by 48 h refeeding increased rat hypothalamic monoamine oxidase (MAO) activity. However, in the interscapular brown adipose tissue (IBAT), only refeeding induced a significant elevation of the enzyme activity. As far as hypothalamic antioxidative enzymes are conc...

journal_title：General physiology and biophysics

authors： Cvijic G,Djordjevic J,Davidovic V

更新日期：2000-09-01 00:00:00

abstract：:Lignin, the structural polymer of the plant cell walls, is produced by free radical polymerization of phenolic alcohols, catalyzed by different peroxidases. The mechanism and the structural organization of lignin in the cell have not been completely understood. In this study we applied fractal analysis to images of li...

journal_title：General physiology and biophysics

authors： Radotić K,Tasić M,Jeremić M,Budimlija Z,Simić-Krstić J,Polzović A,Bozović Z

更新日期：2000-06-01 00:00:00

abstract：:The aim of the present study was to investigate the ability of the chloroacetanilide herbicide acetochlor to interact with the endocrine system. The modulation of the binding of [3H]estradiol-17beta to protamine sulphate-precipitated uterine nuclear and cytoplasmic estrogen receptors was analysed for this purpose. Two...

journal_title：General physiology and biophysics

authors： Rollerová E,Gáspárová Z,Wsólová L,Urbancíková M

更新日期：2000-03-01 00:00:00

abstract：:Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...

journal_title：General physiology and biophysics

authors： Klobusická M,Babusíková O

更新日期：1999-12-01 00:00:00

abstract：:The present results suggest that laminin-1 and 3 are localized in the specialized Schwann cells of Pacinian corpuscles, in spite of incomplete deposition of the basal lamina on the surface of their cytoplasmic processes. In addition, laminin-3 is concentrated and probably function as a stop protein not only in the neu...

journal_title：General physiology and biophysics

authors： Dubový P,Svízenská I,Jancálek R,Klusáková I

更新日期：1999-12-01 00:00:00

abstract：:Apoptosis (programmed cell death) is an important process participating in the formation of organs and tissues during embryogenesis. Our aim of the work is studying the role of the apoptosis during the human embryonic differentiation. We tend to give acquired findings into the correlation with expression of proteins B...

journal_title：General physiology and biophysics

authors： Lichnovský V,Procházková J,Erdösová B,Nepozitková D,Kolár Z

更新日期：1999-12-01 00:00:00

abstract：:The aim of the present work was to test the sensitivity of young male Wistar rats, Dobrá Voda (Dv:WI) to the diabetogenic effect of streptozotocin (STZ) with regard to their health condition and mortality rates. Eight-week-old rats, weighing from 200 to 230 g, were randomised into five groups of eight animals. Strepto...

journal_title：General physiology and biophysics

authors： Gajdosík A,Gajdosíková A,Stefek M,Navarová J,Hozová R

更新日期：1999-10-01 00:00:00

abstract：:On the basis of values corresponding to concentrations exhibiting 50% inhibition of platelet aggregation induced with different stimuli and 50% inhibition of arachidonic acid liberation and thromboxane generation, we compared the antiplatelet effect of two cationic amphiphilic drugs--chloroquine and dithiaden. Compare...

journal_title：General physiology and biophysics

authors： Nosál R,Jancinová V

更新日期：1999-10-01 00:00:00

abstract：:A number of computational methods for the description of the ligand-receptor interaction that were developed in the past decade are reviewed in this paper. The central two sections introduce the methods that are already established as useful tools for the qualitative and quantitative description of ligand-receptor com...

journal_title：General physiology and biophysics

authors： Hornák V,Dvorský R,Sturdík E

更新日期：1999-09-01 00:00:00

abstract：:The passive mechanical properties of whole muscle in active and nonactive states are compared. The experimental results are presented as stress-strain curves, which are analyzed in the framework of the current theoretical background [viz. the freely-jointed chain model (FJCM) and the worm-like chain model (WLCM)] in a...

journal_title：General physiology and biophysics

authors： Muñiz J,Marín JL,Huerta M,Del Rio J,Larios A,Trujillo X

更新日期：1999-06-01 00:00:00

abstract：:Phosphorylation of P-glycoprotein (PGP) by some protein kinases may play an important role in the regulation of its drug transport activity, and may also be important for the development of multidrug resistance (MDR) phenotype. In the present study we investigated the expression of three groups of mitogen-activated pr...

journal_title：General physiology and biophysics

authors： Barancík M,Bohácová V,Zbýnovcová M,Breier A

更新日期：1999-03-01 00:00:00